Chamomile (Matricaria chamomilla L.) is a well-known medicinal plant of great economic value. The purpose of cultivation is the flower heads (Chamomillae flos), which are used as raw materials in the food, pharmaceutical and cosmetic industries. Therefore, chamomile grown in an ecological production system, without the use of pesticides and mineral fertilizers, is extremely valued today. In order to optimize production, it is of utmost importance to assess when, how much and which nutrients to add to the plant. In addition, it is important to investigate how fertilization affects the amount of essential oil, since this is one of the most important parameters in assessing the quality of chamomile. This paper investigated the impact of several types of organic pelleted and granular fertilizers on the yield of dried chamomile flowers and the amount of essential oil, and the obtained data were processed using analysis of variance in the computer program Statistica. The results of the research show that there are statistically significant differences in the yield of dried chamomile flowers between individual fertilization variants, while the difference in the amount of essential oil in flower heads (ml/100g dry matter) is not statistically significant. The profitability of chamomile production in organic farming was also confirmed, since profit was achieved in all fertilization variants.