In the spring of 2022, an experiment with perennial ryegrass (cultivar Maja) was established on experimental plots of the Križevci University of Applied Sciences. The experiment was set up in 6 variants, 5 variants on which ryegrass seeds were coated with polymer emulsion (Flobond SC 101), biostimulators BPH 04 and BSH 05, two variants in which Flobond SC 100 was combined with one of the bacteria and a control variant. Flobond SC 101 is a polymer, a by-product in the production of oil derivatives. BPH 04 is the bacterium Bacillus pulminis, and BSH 05 is the bacterium Bacillus subtilis. The experiment was set up according to a random block arrangement in 4 repetitions. The aim of the research was to determine the influence of polymers, biostimulators and their combinations on the normalized difference vegetation index (NDVI) and the yield of fresh voluminous mass and dry matter of English ryegrass (t/ha). The research lasted two years (2022, 2023). The first year of the research was characterized by a severe drought in March and the summer months, which resulted in significantly lower yields, both fresh mass and dry matter, in 2022 compared to 2023. The yields were in accordance with the NDVI index, which was the lowest in in the 2nd cut of 2022 (on average 0.60), while in the other cuts it ranged from 0.80 to 0.92, lower in 2023. The lowest yields, both fresh mass and dry matter, in all cuts in both years, were recorded on the control variant. The greatest increase in yield, both fresh mass and dry matter, compared to the control variant was achieved with combinations of polymer emulsion and one of the biostimulators, more often BSH 05, then with biostimulator BSH 05, and the least with Flobond SC101, with exceptions in some swaths. As a final conclusion, it can be stated that the treatments had a positive effect on the yield of fresh mass and dry matter of Perennial ryegrass, with an emphasis on the most positive influence of the combination of polymer emulsion and one of the biostimulators. The two investigated years were climatically different, which certainly affected the results.